MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:

Arabidopsis thaliana col: NAD+-dependent isocitrate dehydrogenaseInferred from experiment: IDH-V, IDH-II
Homo sapiens: isocitrate dehydrogenase (NAD)Inferred from experiment: IDH3G, IDH3B, IDH3A
Saccharomyces cerevisiae: isocitrate dehydrogenase [NAD]Inferred from experiment: IDH1, IDH2

In Pathway: TCA cycle II (plants and fungi), L-glutamine biosynthesis III, TCA cycle III (animals)

Reaction Locations: mitochondrial lumen, cytosol

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: isocitrate dehydrogenase (NAD+)

Enzyme Commission Synonyms: isocitric dehydrogenase, β-ketoglutaric-isocitric carboxylase, isocitric acid dehydrogenase, NAD dependent isocitrate dehydrogenase, NAD isocitrate dehydrogenase, NAD-linked isocitrate dehydrogenase, NAD-specific isocitrate dehydrogenase, NAD isocitric dehydrogenase, isocitrate dehydrogenase (NAD), IDH (ambiguous), nicotinamide adenine dinucleotide isocitrate dehydrogenase

Standard Gibbs Free Energy (ΔrG in kcal/mol): 1.6241455Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
Requires Mn2+ or Mg2+ for activity. Unlike EC, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD(+)-linked enzyme ( EC, which is found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP(+)-linked enzyme ( EC that is found in both mitochondria and cytoplasm [Camacho95]. The enzyme from some species can also use NADP+ but much more slowly [Inoue02]. The NADP-dependent enzyme from some species can also use NAD(+), but much more slowly.

Unlike EC, The NAD-dependent enzyme can not use oxalosuccinate as an alternative substrate.

Citations: [Hathaway63, Kornberg51, Plaut54, Vickery62, Kim99c]

Gene-Reaction Schematic

Gene-Reaction Schematic

Unification Links: KEGG:R00709, UniProt:O13696, UniProt:O43837, UniProt:O65501, UniProt:O65852, UniProt:O82004, UniProt:P41563, UniProt:P41564, UniProt:P41565, UniProt:P41566, UniProt:P50213, UniProt:P56472, UniProt:Q7M3F1, UniProt:Q9USP8, UniProt:Q28479, UniProt:Q28480

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:O13696, UniProt:RELATED-TO:O43837, UniProt:RELATED-TO:O65501, UniProt:RELATED-TO:O65852, UniProt:RELATED-TO:O82004, UniProt:RELATED-TO:P28241, UniProt:RELATED-TO:P28834, UniProt:RELATED-TO:P41563, UniProt:RELATED-TO:P41564, UniProt:RELATED-TO:P41565, UniProt:RELATED-TO:P41566, UniProt:RELATED-TO:P50213, UniProt:RELATED-TO:P56472, UniProt:RELATED-TO:Q7M3F1, UniProt:RELATED-TO:Q9USP8, UniProt:RELATED-TO:Q28479, UniProt:RELATED-TO:Q28480

Revised 13-Jun-2011 by Caspi R, SRI International


Camacho95: Camacho ML, Brown RA, Bonete MJ, Danson MJ, Hough DW (1995). "Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence." FEMS Microbiol Lett 134(1);85-90. PMID: 8593959

Hathaway63: Hathaway, JA, Atkinson, DE (1963). "The effect of adenylic acid on yeast nicotinamide adenine dinucleotide isocitrate dehydrogenase, a possible metabolic control mechanism." J Biol Chem 238;2875-81. PMID: 14063317

Inoue02: Inoue H, Tamura T, Ehara N, Nishito A, Nakayama Y, Maekawa M, Imada K, Tanaka H, Inagaki K (2002). "Biochemical and molecular characterization of the NAD(+)-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans." FEMS Microbiol Lett 214(1);127-32. PMID: 12204383

Kim99c: Kim YO, Koh HJ, Kim SH, Jo SH, Huh JW, Jeong KS, Lee IJ, Song BJ, Huh TL (1999). "Identification and functional characterization of a novel, tissue-specific NAD(+)-dependent isocitrate dehydrogenase beta subunit isoform." J Biol Chem 274(52);36866-75. PMID: 10601238

Kornberg51: Kornberg, A, Pricer, WE (1951). "Di- and triphosphopyridine nucleotide isocitric dehydrogenases in yeast." J Biol Chem 189(1);123-36. PMID: 14832224

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Plaut54: Plaut, GW, Sung, SC (1954). "Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues." J Biol Chem 207(1);305-14. PMID: 13152105

Vickery62: Vickery, HB (1962). "A suggested new nomenclature for the isomers of isocitric acid." J Biol Chem 237;1739-41. PMID: 13925783

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Mon Nov 30, 2015, BIOCYC11A.