MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:,

Enzymes and Genes:

Arabidopsis thaliana col:
Bacillus subtilis: UTP-glucose-1-phosphate uridylyltransferaseInferred from experiment: gtaB
Cucumis melo:
Escherichia coli K-12 substr. MG1655: UTP--glucose-1-phosphate uridylyltransferaseInferred from experiment: galU
Haloferax volcanii: UTP--glucose-1-phosphate uridylyltransferaseInferred from experiment: aglF
Homo sapiens: UDP-glucose pyrophosphorylaseInferred from experiment: UGP2
Leishmania major: UDP-sugar pyrophosphorylaseInferred from experiment: USP
Mus musculus: UDP-glucose pyrophosphorylase 2Inferred by curatorInferred from experiment: Ugp2
Mycoplasma pneumoniae M129: UDP-glucose pyrophosphorylaseInferred from experiment: galU
Pisum sativum: UDP-D-glucose pyrophosphorylaseInferred from experiment: USP
Saccharomyces cerevisiae: UDP-glucose pyrophosphorylaseInferred from experiment: UGP1

In Pathway: stachyose degradation, sucrose biosynthesis II, sucrose degradation II (sucrose synthase), UDP-glucose biosynthesis

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name for UTP—glucose-1-phosphate uridylyltransferase

Enzyme Commission Synonyms for UDP glucose pyrophosphorylase, glucose-1-phosphate uridylyltransferase, UDPG phosphorylase, UDPG pyrophosphorylase, uridine 5'-diphosphoglucose pyrophosphorylase, uridine diphosphoglucose pyrophosphorylase, uridine diphosphate-D-glucose pyrophosphorylase, uridine-diphosphate glucose pyrophosphorylase

Enzyme Commission Primary Name for UTP-monosaccharide-1-phosphate uridylyltransferase

Enzyme Commission Synonyms for UDP-sugar pyrophosphorylase, PsUSP

Standard Gibbs Free Energy (ΔrG in kcal/mol): -103.30347Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary for
The reaction can occur in either direction and it has been postulated that MgUTP and Mg-diphosphate are the actual substrates. Catalyzes the formation of UDP-Glc, UDP-Gal, UDP-GlcA, UDP-L-Ara and UDP-Xyl, showing broad substrate specificity toward monosaccharide 1-phosphates. Mannose 1-phosphate, L-fucose 1-phosphate and glucose 6-phosphate are not substrates and UTP cannot be replaced by other nucleotide triphosphates.

Citations: [Dai06a, LobelleRich83, Kamogawa65, KALCKAR, SMITH55, Turnquist74, Rudick74, Kotake04]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reactions of [UTP + a sugar 1-phosphate + H+ ↔ a UDP-sugar + diphosphate] (
i1: α-D-galactose 1-phosphate + UTP + H+ ↔ UDP-α-D-galactose + diphosphate (

i2: α-D-glucopyranose 1-phosphate + UTP + H+ ↔ UDP-α-D-glucose + diphosphate (
i3: UTP + N-acetyl-α-D-galactosamine 1-phosphate + H+ = UDP-N-acetyl-α-D-galactosamine + diphosphate (

i4: N-acetyl-α-D-glucosamine 1-phosphate + UTP + H+ → UDP-N-acetyl-α-D-glucosamine + diphosphate (

i5: α-D-xylose 1-phosphate + UTP + H+ ↔ UDP-α-D-xylose + diphosphate (

Unification Links: KEGG:R00289, PIR:A40650, PIR:A41382, PIR:A56146, PIR:A64250, PIR:A64970, PIR:A75096, PIR:D49349, PIR:D69184, PIR:D70601, PIR:E64466, PIR:E71913, PIR:F59102, PIR:F64600, PIR:F81300, PIR:G64095, PIR:G70125, PIR:H70446, PIR:JC2265, PIR:JC4785, PIR:JC4985, PIR:JX0277, PIR:S15298, PIR:S30007, PIR:S31431, PIR:S35692, PIR:S41533, PIR:S62599, PIR:S73501, PIR:S78541, PIR:T42521, PIR:T44841, PIR:T45453, PIR:XNDOU, PIR:XNPOU, Rhea:19889, UniProt:O05576, UniProt:O25363, UniProt:O26731, UniProt:O51225, UniProt:O67602, UniProt:P08800, UniProt:P0AAB6, UniProt:P0AEP3, UniProt:P19595, UniProt:P27897, UniProt:P32861, UniProt:P33696, UniProt:P37776, UniProt:P44878, UniProt:P47691, UniProt:P74969, UniProt:P75124, UniProt:P78811, UniProt:Q9PMD3, UniProt:Q9RMC3, UniProt:Q9UZI7, UniProt:Q9X364, UniProt:Q9Z5G1, UniProt:Q9ZLI8, UniProt:Q05852, UniProt:Q07130, UniProt:Q07131, UniProt:Q43772, UniProt:Q46768, UniProt:Q48447, UniProt:Q58730

Relationship Links: BRENDA:EC:, BRENDA:EC:, ENZYME:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, IUBMB-ExplorEnz:EC:, UniProt:RELATED-TO:O05576, UniProt:RELATED-TO:O25363, UniProt:RELATED-TO:O26731, UniProt:RELATED-TO:O51225, UniProt:RELATED-TO:O67602, UniProt:RELATED-TO:P08800, UniProt:RELATED-TO:P0AAB6, UniProt:RELATED-TO:P0AEP3, UniProt:RELATED-TO:P19595, UniProt:RELATED-TO:P27897, UniProt:RELATED-TO:P32861, UniProt:RELATED-TO:P33696, UniProt:RELATED-TO:P37776, UniProt:RELATED-TO:P44878, UniProt:RELATED-TO:P47691, UniProt:RELATED-TO:P74969, UniProt:RELATED-TO:P75124, UniProt:RELATED-TO:P78811, UniProt:RELATED-TO:Q9PMD3, UniProt:RELATED-TO:Q9RMC3, UniProt:RELATED-TO:Q9UZI7, UniProt:RELATED-TO:Q9X364, UniProt:RELATED-TO:Q9Z5G1, UniProt:RELATED-TO:Q9ZLI8, UniProt:RELATED-TO:Q05852, UniProt:RELATED-TO:Q07130, UniProt:RELATED-TO:Q07131, UniProt:RELATED-TO:Q43772, UniProt:RELATED-TO:Q46768, UniProt:RELATED-TO:Q48447, UniProt:RELATED-TO:Q58730

Created 18-Jun-2010 by Pujar A, Boyce Thompson Institute


Dai06a: Dai N, Petreikov M, Portnoy V, Katzir N, Pharr DM, Schaffer AA (2006). "Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants." Plant Physiol 142(1);294-304. PMID: 16829585

KALCKAR: KALCKAR HM "The role of phosphoglycosyl compounds in the biosynthesis of nucleosides and nucleotides." Biochim Biophys Acta 12(1-2);250-64. PMID: 13115434

Kamogawa65: Kamogawa A, Kurahashi K (1965). "Purification and properties of uridinediphosphate glucose pyrophosphorylase from Escherichia coli K12." J Biochem 57(6);758-65. PMID: 4284510

Kotake04: Kotake T, Yamaguchi D, Ohzono H, Hojo S, Kaneko S, Ishida HK, Tsumuraya Y (2004). "UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts." J Biol Chem 279(44);45728-36. PMID: 15326166

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

LobelleRich83: Lobelle-Rich PA, Reeves RE (1983). "Separation and characterization of two UTP-utilizing hexose phosphate uridylyltransferases from Entamoeba histolytica." Mol Biochem Parasitol 7(2);173-82. PMID: 6304512

Rudick74: Rudick VL, Weisman RA (1974). "Uridine diphosphate glucose pyrophosphorylase of Acanthamoeba castellanii. Purification, kinetic, and developmental studies." J Biol Chem 249(24);7832-40. PMID: 4430676

SMITH55: SMITH EE, MILLS GT (1955). "The uridyl transferase of mammary gland." Biochim Biophys Acta 18(1);152. PMID: 13260264

Turnquist74: Turnquist RL, Gillett TA, Hansen RG (1974). "Uridine diphosphate glucose pyrophosphorylase. Crystallization and properties of the enzyme from rabbit liver and species comparisons." J Biol Chem 249(23);7695-700. PMID: 4436332

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Thu Nov 26, 2015, BIOCYC14A.