Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015
Metabolic Modeling Tutorial
early discounted registration
ends Feb 21th, 2015

MetaCyc Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:
cysteine synthase B Inferred from experiment : cysM ( Escherichia coli K-12 substr. MG1655 )
O-acetylserine sulfhydrylase A Inferred from experiment : cysK ( Escherichia coli K-12 substr. MG1655 )
O-ureido-L-serine synthase Inferred from experiment : dcsD ( Streptomyces lavendulae lavendulae )
cysteine synthase Inferred from experiment ( Astragalus bisulcatus )
O-acetylserine (thiol) lyase Inferred from experiment : oasC ( Arabidopsis thaliana col )
O-acetylserine (thiol) lyase Inferred from experiment : oasB ( Arabidopsis thaliana col )
O-acetylserine (thiol) lyase Inferred from experiment : oasA1 ( Arabidopsis thaliana col )

In Pathway: cysteine biosynthesis I

Supersedes EC number:

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: cysteine synthase

Enzyme Commission Synonyms: O-acetyl-L-serine sulfhydrylase, O-acetyl-L-serine sulfohydrolase, O-acetylserine (thiol)-lyase, O-acetylserine (thiol)-lyase A, O-acetylserine sulfhydrylase, O3-acetyl-L-serine acetate-lyase (adding hydrogen-sulfide), acetylserine sulfhydrylase, cysteine synθse, S-sulfocysteine synthase, 3-O-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase, O3-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

Standard Gibbs Free Energy (ΔrG in kcal/mol): -11.374123 Inferred by computational analysis [Latendresse13]

Enzyme Commission Summary:
A pyridoxal-phosphate protein. Some alkyl thiols, cyanide, pyrazole and some other heterocyclic compounds can act as acceptors. Not identical with EC, β-pyrazolylalanine synthase, EC, L-mimosine synthase and EC, uracilylalanine synthase.

Citations: [Becker69, Hara90, Tai01, Bettati00, Ikegami87, Murakoshi86]

Gene-Reaction Schematic: ?

Unification Links: KEGG:R00897 , Rhea:14829

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC: , UniProt:RELATED-TO:O05393 , UniProt:RELATED-TO:O32978 , UniProt:RELATED-TO:O34476 , UniProt:RELATED-TO:O45679 , UniProt:RELATED-TO:O67507 , UniProt:RELATED-TO:O81154 , UniProt:RELATED-TO:O81155 , UniProt:RELATED-TO:O81523 , UniProt:RELATED-TO:P0A1E3 , UniProt:RELATED-TO:P0A534 , UniProt:RELATED-TO:P0ABK5 , UniProt:RELATED-TO:P16703 , UniProt:RELATED-TO:P29848 , UniProt:RELATED-TO:P31300 , UniProt:RELATED-TO:P32260 , UniProt:RELATED-TO:P37887 , UniProt:RELATED-TO:P38076 , UniProt:RELATED-TO:P45040 , UniProt:RELATED-TO:P47998 , UniProt:RELATED-TO:P47999 , UniProt:RELATED-TO:P53206 , UniProt:RELATED-TO:P56067 , UniProt:RELATED-TO:P63873 , UniProt:RELATED-TO:P71128 , UniProt:RELATED-TO:P80608 , UniProt:RELATED-TO:P87131 , UniProt:RELATED-TO:Q7M1J2 , UniProt:RELATED-TO:Q93244 , UniProt:RELATED-TO:Q9CHF0 , UniProt:RELATED-TO:Q9CI26 , UniProt:RELATED-TO:Q9JQL6 , UniProt:RELATED-TO:Q9RAS8 , UniProt:RELATED-TO:Q9RW80 , UniProt:RELATED-TO:Q9S2H1 , UniProt:RELATED-TO:Q9S6Z7 , UniProt:RELATED-TO:Q9S757 , UniProt:RELATED-TO:Q9SMY4 , UniProt:RELATED-TO:Q9UWP0 , UniProt:RELATED-TO:Q9V1Q3 , UniProt:RELATED-TO:Q9WZD3 , UniProt:RELATED-TO:Q9XDU7 , UniProt:RELATED-TO:Q9ZMW6 , UniProt:RELATED-TO:Q00834 , UniProt:RELATED-TO:Q43153 , UniProt:RELATED-TO:Q43317 , UniProt:RELATED-TO:Q43725 , UniProt:RELATED-TO:Q43726 , UniProt:RELATED-TO:Q59447 , UniProt:RELATED-TO:Q59529

Revised 10-Oct-2011 by Caspi R , SRI International


Becker69: Becker MA, Kredich NM, Tomkins GM (1969). "The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium." J Biol Chem 244(9);2418-27. PMID: 4891157

Bettati00: Bettati S, Benci S, Campanini B, Raboni S, Chirico G, Beretta S, Schnackerz KD, Hazlett TL, Gratton E, Mozzarelli A (2000). "Role of pyridoxal 5'-phosphate in the structural stabilization of O-acetylserine sulfhydrylase." J Biol Chem 275(51);40244-51. PMID: 10995767

Hara90: Hara S, Payne MA, Schnackerz KD, Cook PF (1990). "A rapid purification procedure and computer-assisted sulfide ion selective electrode assay for O-acetylserine sulfhydrylase from Salmonella typhimurium." Protein Expr Purif 1(1);70-6. PMID: 2152186

Ikegami87: Ikegami F, Kaneko M, Lambein F, Kuo Y-H, Murakoshi I (1987). "Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum." Phytochemistry 26 2699-2704.

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Murakoshi86: Murakoshi I, Kaneko M, Koide C, Ikegami F (1986). "Enzymatic-synthesis of the neuroexcitatory amino-acid quisqualic by cysteine synthase." Phytochemistry 25 2759-2763.

Tai01: Tai CH, Burkhard P, Gani D, Jenn T, Johnson C, Cook PF (2001). "Characterization of the allosteric anion-binding site of O-acetylserine sulfhydrylase." Biochemistry 40(25);7446-52. PMID: 11412097

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Tue Mar 3, 2015, biocyc11.