|Gene:||crtI||Accession Number: G-11381 (MetaCyc)|
Species: Rhodobacter capsulatus
The crtI gene from Rhodobacter capsulatus was found to be responsible for this activity [Giuliano86]. The gene was cloned and expressed in Escherichia coli, and the resulting protein was purified [Raisig96]. The enzyme converted all-trans-phytoene, as well as the intermediates all-trans phytofluene and all-trans-ζ-carotene, to all-trans neurosporene, the end product of the reaction.
1,2-epoxy phytoene was also a suitable substrate.
Gene Citations: [Bartley89]
Accession Numbers : EG10336 (EcoCyc)
|. FruA lacking the B' domain shows less affinity for FruB in vitro. Loss of the B' domain mainly affects phosphoryl transfer between FruB which contains the pseudo-HPr domain and FruA Enzymatic reaction of: ζ-carotene desaturase (phytoene desaturase)|
[ onMouseOver="return overlib(' Charbit A, Reizer J, Saier MH (1996). "Function of the duplicated IIB domain and oligomeric structure of the fructose permease of Escherichia coli." J Biol Chem 271(17);9997-10003. PMID: 8626640', WIDTH, 500);" onmouseout="return nd();">Charbit96].
This subunit contains PTS Enzyme IIB', IIB and IIC domains.
The crystal structure of this subunit has been resolved to 2.6 Å [
a reduced electron acceptor
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated. The reaction is favored in the direction shown. In Pathways: neurosporene biosynthesis
Cofactors or Prosthetic Groups: FAD
[Raisig96] Inhibitors (Unknown Mechanism): diphenylamine
|Enzymatic reaction of: phytofluene desaturase (phytoene desaturase)|
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