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MetaCyc Enzyme: 2-keto-3-deoxy-L-galactonate aldolase

Gene: LGA1 Accession Number: G-11976 (MetaCyc)

Synonyms: kdg1

Species: Trichoderma reesei

Summary:
The subunit structure of this enzyme from Trichoderma reesei (previously known as Hypocrea jecorina) has not been reported.

The enzyme activity was induced by growth of the organism on D-galacturonate [Hilditch07].

Recombinant enzyme was expressed in Saccharomyces cerevisiae as a His-tagged protein, purified and characterized [Hilditch07].
A putative ortholog of this gene was identified in Aspergillus niger, designated GAAC [MartensUzunova08].

Molecular Weight of Polypeptide: 33.407 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:EF203091 , Protein Model Portal:A6Y9S5 , String:51453.JGI60067 , UniProt:A6Y9S5

Relationship Links: InterPro:IN-FAMILY:IPR002220 , InterPro:IN-FAMILY:IPR013785 , Panther:IN-FAMILY:PTHR12128 , Pfam:IN-FAMILY:PF00701 , Prints:IN-FAMILY:PR00146

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 15-Apr-2010 by Fulcher CA , SRI International


Enzymatic reaction of: 2-keto-3-deoxy-L-galactonate aldolase

Synonyms: 2-keto-3-deoxy-L-galactonic acid aldolase, L-threo-3-deoxy-hexulosonate aldolase

EC Number: 4.1.2.54

2-dehydro-3-deoxy-L-galactonate <=> L-glyceraldehyde + pyruvate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Hilditch07]

Alternative Substrates for L-glyceraldehyde: glycolaldehyde [Hilditch07 ] , methylglyoxal [Hilditch07 ]

In Pathways: superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galacturonate degradation III

Kinetic Parameters:

Substrate
Km (μM)
Citations
2-dehydro-3-deoxy-L-galactonate
3500.0
[Hilditch07]
pyruvate
500.0
[Hilditch07]
L-glyceraldehyde
1200.0
[Hilditch07]


References

Hilditch07: Hilditch S, Berghall S, Kalkkinen N, Penttila M, Richard P (2007). "The missing link in the fungal D-galacturonate pathway: identification of the L-threo-3-deoxy-hexulosonate aldolase." J Biol Chem 282(36);26195-201. PMID: 17609199

MartensUzunova08: Martens-Uzunova ES, Schaap PJ (2008). "An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation." Fungal Genet Biol 45(11);1449-57. PMID: 18768163

Richard09: Richard P, Hilditch S (2009). "D-galacturonic acid catabolism in microorganisms and its biotechnological relevance." Appl Microbiol Biotechnol 82(4);597-604. PMID: 19159926


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Mar 30, 2015, biocyc14.