MetaCyc Enzyme: 2-keto-3-deoxy-L-galactonate aldolase

Gene: LGA1 Accession Number: G-11976 (MetaCyc)

Synonyms: kdg1

Species: Trichoderma reesei

The subunit structure of this enzyme from Trichoderma reesei (previously known as Hypocrea jecorina) has not been reported.

The enzyme activity was induced by growth of the organism on D-galacturonate [Hilditch07].

Recombinant enzyme was expressed in Saccharomyces cerevisiae as a His-tagged protein, purified and characterized [Hilditch07].
A putative ortholog of this gene was identified in Aspergillus niger, designated GAAC [MartensUzunova08].

Molecular Weight of Polypeptide: 33.407 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:EF203091 , Protein Model Portal:A6Y9S5 , String:51453.JGI60067 , UniProt:A6Y9S5

Relationship Links: InterPro:IN-FAMILY:IPR002220 , InterPro:IN-FAMILY:IPR013785 , Panther:IN-FAMILY:PTHR12128 , Pfam:IN-FAMILY:PF00701 , Prints:IN-FAMILY:PR00146

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 15-Apr-2010 by Fulcher CA , SRI International

Enzymatic reaction of: 2-keto-3-deoxy-L-galactonate aldolase

Synonyms: 2-keto-3-deoxy-L-galactonic acid aldolase, L-threo-3-deoxy-hexulosonate aldolase

EC Number:

2-dehydro-3-deoxy-L-galactonate <=> L-glyceraldehyde + pyruvate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Hilditch07]

Alternative Substrates for L-glyceraldehyde: glycolaldehyde [Hilditch07 ] , methylglyoxal [Hilditch07 ]

In Pathways: superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galacturonate degradation III

Kinetic Parameters:

Km (μM)


Hilditch07: Hilditch S, Berghall S, Kalkkinen N, Penttila M, Richard P (2007). "The missing link in the fungal D-galacturonate pathway: identification of the L-threo-3-deoxy-hexulosonate aldolase." J Biol Chem 282(36);26195-201. PMID: 17609199

MartensUzunova08: Martens-Uzunova ES, Schaap PJ (2008). "An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation." Fungal Genet Biol 45(11);1449-57. PMID: 18768163

Richard09: Richard P, Hilditch S (2009). "D-galacturonic acid catabolism in microorganisms and its biotechnological relevance." Appl Microbiol Biotechnol 82(4);597-604. PMID: 19159926

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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