|Gene:||LGA1||Accession Number: G-11976 (MetaCyc)|
Species: Trichoderma reesei
The subunit structure of this enzyme from Trichoderma reesei (previously known as Hypocrea jecorina) has not been reported.
Recombinant enzyme was expressed in Saccharomyces cerevisiae as a His-tagged protein, purified and characterized [Hilditch07].
A putative ortholog of this gene was identified in Aspergillus niger, designated GAAC [MartensUzunova08].
Molecular Weight of Polypeptide: 33.407 kD (from nucleotide sequence)
Enzymatic reaction of: 2-keto-3-deoxy-L-galactonate aldolase
Synonyms: 2-keto-3-deoxy-L-galactonic acid aldolase, L-threo-3-deoxy-hexulosonate aldolase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible. [Hilditch07]
Hilditch07: Hilditch S, Berghall S, Kalkkinen N, Penttila M, Richard P (2007). "The missing link in the fungal D-galacturonate pathway: identification of the L-threo-3-deoxy-hexulosonate aldolase." J Biol Chem 282(36);26195-201. PMID: 17609199
MartensUzunova08: Martens-Uzunova ES, Schaap PJ (2008). "An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation." Fungal Genet Biol 45(11);1449-57. PMID: 18768163
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493