Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
twitter

MetaCyc Transporter: predicted PTS permease CmtBA

Synonyms: EIICmt, Enzyme IICmt

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of predicted PTS permease CmtBA = [CmtB][CmtA]
         predicted PTS permease - CmtB subunit = CmtB (summary available)
         predicted PTS permease - CmtA subunit = CmtA (summary available)

Summary:
CmtBA, the "cryptic mannitol" PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93]). cmtA and cmtB may encode a mannitol-like PTS permease. The two genes complement a mannitol-negative E. coli mutant when expressed using a heterologous promoter [Sprenger93]. The cmt genes have a lower G+C content than the mtlA operon [Sprenger93].

The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTS, Enzyme I (PtsI) and HPr (PtsH), as well as the three domains of the Enzyme IICmt complex is:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIA-Phis67 → Enzyme IIB-Pcys377 - (Enzyme IIC) → sugar-P

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Biological Process:
Inferred by computational analysisInferred from experimentGO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system [Sprenger93]

Credits:
Imported from EcoCyc 30-Sep-2015 by Paley S, SRI International


Enzymatic reaction of: D-mannitol transport (predicted PTS permease CmtBA)

Inferred from experimentInferred by computational analysis

Transport reaction diagram for D-mannitol transport

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.

Credits:
Imported from EcoCyc 30-Sep-2015 by Paley S, SRI International

Kinetic Parameters:
Substrate Km (μM) Citations
D-mannitol 5000.0 [Begley96a, BRENDA14]
D-mannitol 11.0 [Jacobson83, BRENDA14]

pH(opt): 6 [BRENDA14, Saier80], 6.5 [BRENDA14, Jacobson83], 7 [BRENDA14, Jacobson79], 7.5 [BRENDA14, Sutrina87], 8.5 [BRENDA14, Jacobson83], 9 [BRENDA14, Jacobson79], 9.5 [BRENDA14, Saier80]


Subunit of predicted PTS permease CmtBA: predicted PTS permease - CmtB subunit

Synonyms: TolM, Enzyme IIACmt, EIIACmt, Enzyme IIICmt

Gene: cmtB Accession Numbers: EG11791 (MetaCyc), b2934, ECK2929

Locations: cytosol

Sequence Length: 147 AAs

Molecular Weight: 16.046 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0008643 - carbohydrate transport [UniProtGOA11a]
Inferred by computational analysisGO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system [UniProtGOA11a, Sprenger93]
Inferred by computational analysisGO:0016310 - phosphorylation [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Inferred by computational analysisGO:0016301 - kinase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016740 - transferase activity [UniProtGOA11a]
Cellular Component:
Inferred by computational analysisGO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a, Sprenger93]
Inferred by computational analysisGO:0005829 - cytosol [DiazMejia09]

MultiFun Terms: transportGroup TranslocatorsPhosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b2934, ModBase:P69824, PR:PRO_000022306, Protein Model Portal:P69824, RefSeq:NP_417409, SMR:P69824, UniProt:P69824

Relationship Links: InterPro:IN-FAMILY:IPR002178, InterPro:IN-FAMILY:IPR016152, PDB:Structure:2OQ3, Pfam:IN-FAMILY:PF00359, Prosite:IN-FAMILY:PS00372, Prosite:IN-FAMILY:PS51094

Summary:
cmtB encodes a hydrophilic protein with seqence similarity to the EIIA domain of MltA, the mannitol PTS permease [Sprenger93]. Expression of cmtB from a lac promoter partially complements the growth defect of an E. coli mtlA C-terminal deletion mutant [Sprenger93]. A solution structure of CmtB shows high similarity with that of the MltA IIA domain although structural differences around the active site are present. Histidine residue 67 is predicted to be the site of phosphorylation [Yu07].


Subunit of predicted PTS permease CmtBA: predicted PTS permease - CmtA subunit

Synonyms: EIICBCmt, Enzyme IICBCmt

Gene: cmtA Accession Numbers: EG11792 (MetaCyc), b2933, ECK2928

Locations: inner membrane

Sequence Length: 462 AAs

Molecular Weight: 48.971 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0008643 - carbohydrate transport [UniProtGOA11a]
Inferred by computational analysisGO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system [UniProtGOA11a, GOA01a, Sprenger93]
Inferred by computational analysisGO:0015797 - mannitol transport [GOA01a]
Inferred by computational analysisGO:0016310 - phosphorylation [UniProtGOA11a]
Inferred by computational analysisGO:0034219 - carbohydrate transmembrane transport [GOA01, GOA01a]
Molecular Function:
Inferred by computational analysisGO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity [GOA01, GOA01a]
Inferred by computational analysisGO:0016301 - kinase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016740 - transferase activity [UniProtGOA11a]
Inferred by computational analysisGO:0022872 - protein-N(PI)-phosphohistidine-mannitol phosphotransferase system transmembrane transporter activity [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0005887 - integral component of plasma membrane [Sprenger93]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a, GOA01a]

MultiFun Terms: transportGroup TranslocatorsPhosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b2933, PR:PRO_000022305, Protein Model Portal:P69826, RefSeq:NP_417408, SMR:P69826, Swiss-Model:P69826, UniProt:P69826

Relationship Links: InterPro:IN-FAMILY:IPR003352, InterPro:IN-FAMILY:IPR003501, InterPro:IN-FAMILY:IPR004718, InterPro:IN-FAMILY:IPR013011, InterPro:IN-FAMILY:IPR013014, InterPro:IN-FAMILY:IPR029503, Pfam:IN-FAMILY:PF02302, Pfam:IN-FAMILY:PF02378, Prosite:IN-FAMILY:PS51099, Prosite:IN-FAMILY:PS51104

Summary:
CmtA has 52% amino acid identity with the EIICB domain of MltA, the mannitol PTS permease. cmtA may encode a protein with 6 transmembrane regions; a conserved cysteine residue at position 377 may be required for phosphoryl transfer to substrate. Expression of cmtA under the control of heterogous promoter led to mannitol transport in mtlA mutants of E. coli K-12 [Sprenger93].


References

Begley96a: Begley GS, Warner KA, Arents JC, Postma PW, Jacobson GR (1996). "Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease." J Bacteriol 178(3);940-2. PMID: 8550539

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jacobson79: Jacobson GR, Lee CA, Saier MH (1979). "Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system." J Biol Chem 254(2);249-52. PMID: 368051

Jacobson83: Jacobson GR, Lee CA, Leonard JE, Saier MH (1983). "Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease." J Biol Chem 258(17);10748-56. PMID: 6350293

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Saier80: Saier MH (1980). "Catalytic activities associated with the enzymes II of the bacterial phosphotransferase system." J Supramol Struct 14(3);281-94. PMID: 7012451

Sprenger93: Sprenger GA (1993). "Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various gram-positive bacteria." Biochim Biophys Acta 1993;1158(1);103-6. PMID: 8353127

Sutrina87: Sutrina SL, Waygood EB, Grenier FC, Saier MH (1987). "HPr/HPr-P phosphoryl exchange reaction catalyzed by the mannitol specific enzyme II of the bacterial phosphotransferase system." J Biol Chem 262(6);2636-41. PMID: 3102473

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yu07: Yu C, Li Y, Xia B, Jin C (2007). "Solution structure of the cryptic mannitol-specific phosphotransferase enzyme IIA CmtB from Escherichia coli." Biochem Biophys Res Commun 362(4);1001-6. PMID: 17803963


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Mon May 2, 2016, biocyc13.