Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

Escherichia coli K-12 substr. MG1655 Enzyme: NMN amidohydrolase



Gene: pncC Accession Numbers: G7409 (EcoCyc), b2700, ECK2695

Synonyms: ygaD

Regulation Summary Diagram: ?

Subunit composition of NMN amidohydrolase = [PncC]2
         NMN aminohydrolase = PncC

Summary:
NMN amidohydrolase takes part in the intracellular recycling of NAD, catalyzing the deamidation of NMN to NaMN [Hillyard81]. The enzyme is in the most active path (PNC IV) for recycling NAD in E. coli [Neidhardt96].

Until recently, the gene encoding NMN amidohydrolase was unknown. Aided by genomics-based reconstruction of NAD metabolism, the gene was finally identified by Galeazzi et al. [Galeazzi11].

PncC: "pyridine nucleotide cycle"

Locations: cytosol

Map Position: [2,821,871 <- 2,822,368] (60.82 centisomes)
Length: 498 bp / 165 aa

Molecular Weight of Polypeptide: 17.582 kD (from nucleotide sequence), 20.0 kD (experimental) [Galeazzi11 ]

Molecular Weight of Multimer: 33.0 kD (experimental) [Hillyard81]

Unification Links: ASAP:ABE-0008882 , DIP:DIP-48189N , EchoBASE:EB2560 , EcoGene:EG12698 , EcoliWiki:b2700 , OU-Microarray:b2700 , PortEco:ygaD , Pride:P0A6G3 , Protein Model Portal:P0A6G3 , RefSeq:NP_417180 , RegulonDB:G7409 , SMR:P0A6G3 , String:511145.b2700 , UniProt:P0A6G3

Relationship Links: InterPro:IN-FAMILY:IPR008136 , Pfam:IN-FAMILY:PF02464

In Paralogous Gene Group: 311 (2 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0019363 - pyridine nucleotide biosynthetic process Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0019159 - nicotinamide-nucleotide amidase activity Inferred from experiment Inferred by computational analysis [GOA01a, Galeazzi11]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism biosynthesis of building blocks cofactors, small molecule carriers nicotinamide adenine dinucleotide

Essentiality data for pncC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Created 30-Sep-2011 by Keseler I , SRI International
Last-Curated ? 03-Oct-2011 by Keseler I , SRI International


Enzymatic reaction of: NMN amidohydrolase

Synonyms: nicotinamide mononucleotide amidohydrolase, nicotinamide-nucleotide amidase, NMN deamidase

EC Number: 3.5.1.42

β-nicotinamide D-ribonucleotide + H2O <=> ammonium + β-nicotinate D-ribonucleotide

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates [Comment 4]:

In Pathways: NAD salvage pathway I

Kinetic Parameters:

Substrate
Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)
Specific Activity (U/mg)
Citations
β-nicotinamide D-ribonucleotide
14.0
[Hillyard81, BRENDA14]
β-nicotinamide D-ribonucleotide
6.0
3.3
[Galeazzi11]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Galeazzi11: Galeazzi L, Bocci P, Amici A, Brunetti L, Ruggieri S, Romine M, Reed S, Osterman AL, Rodionov DA, Sorci L, Raffaelli N (2011). "Identification of nicotinamide mononucleotide deamidase of the bacterial pyridine nucleotide cycle reveals a novel broadly conserved amidohydrolase family." J Biol Chem 286(46);40365-75. PMID: 21953451

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hillyard81: Hillyard D, Rechsteiner M, Manlapaz-Ramos P, Imperial JS, Cruz LJ, Olivera BM (1981). "The pyridine nucleotide cycle. Studies in Escherichia coli and the human cell line D98/AH2." J Biol Chem 1981;256(16);8491-7. PMID: 7021549

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Nonaka06: Nonaka G, Blankschien M, Herman C, Gross CA, Rhodius VA (2006). "Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress." Genes Dev 20(13);1776-89. PMID: 16818608


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc14.